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Additional resources for Blueprints in Cardiology [USMLE Steps 2,3]
Epstein-Barr virus latent membrane protein 1 mediates phosphorylation and nuclear translocation of annexin A2 by activating PKC pathway. Cell Signal. 2007;19:341–8. Kuster B, Wheeler SF, Hunter AP, Dwek RA, Harvey DJ. Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ ionization mass spectrometry and normal-phase high-performance liquid chromatography. Anal Biochem. 1997;250:82–101. Packer NH, Lawson MA, Jardine DR, Sanchez JC, Gooley AA.
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Another approach, Phosphate Metal Affinity Chromatography (PMAC), has a broader spectrum of applications because it allows any phosphorylated protein to be enriched from cellular extracts . Currently, a number of MS/MS-based technologies are available to identify phospho sites on proteins. Most of them require protease-dependent breaking down of protein into peptides to determine the sequence of phosphopeptide(s), whereas FTICR MS is able to identify PTMs at the protein level . Identification of new phosphoprotein(s) as a target(s) of certain signaling cascade requires investigation of the molecular mechanism involved in the regulatory phosphorylation of these proteins.